广州地区儿童感染幽门螺杆菌iceA基因亚型与胃炎的相关性研究

The correlative study of H.Pylori iceA genbtype and gastritis in children in Guangzhou

目的探讨广州地区儿童感染幽门螺杆菌(H.pylori)的iceA基因亚型与胃炎的关系。方法105例胃、十二指肠疾病患儿的胃窦处取3块胃黏膜,分别进行快速尿素酶反应、病理检查和聚合酶链反应(PCR)。抽提胃黏膜基因组DNA,用3对引物检测H.pyloriureA和iceA基因,分析H.pylori iceA基因亚型与胃炎的关系。结果105例样本中,快速尿素酶反应、病理检测和聚合酶链反应(PCR)三者均阳性的标本52例,H.pylori iceA1亚型菌株单独检出率78.84%(41/52),H.pylori iceA2亚型菌株单独检出率1.92%(1/52),H.pylori iceA1和iceA2亚型均阳性的检出率3.84%(2/52),iceA1和iceA2亚型均阴性的比率15.38%(8/52),H.pylori iceA1亚型阳性率与其它基因亚型相比较,差异有显著性;感染H.pylori iceA1亚型菌株的患儿中轻度慢性胃炎3例,中度慢性胃炎15例,重度慢性胃炎25例;未感染H.pylori iceA1亚型菌株的患儿中轻度慢性胃炎2例,中度慢性胃炎4例,重度慢性胃炎3例,感染H.pylori iceA1亚型菌株患儿与未感染H.pylori iceA1亚型菌株患儿的胃黏膜病变程度差异有显著性(P<0.05)。结论H.pylori iceA1亚型是广州地区儿童感染H.pylori的优势基因亚型,H.pylori iceA1菌株容易引起较严重慢性胃炎。

Objective To investigate the correlation of ieeA genotypes of Helieobacter pylori isolated from children in Guangzhou and gastritis. Methods One hundred and five children were endescoped in Guangzhou Children＇s Hospital. For each patient, 3 biopsy specimens were taken from the gastric antrum, with one for rapid urease reaction,one for histological examination, and one for pelymerase chain reaction（PCR） for detecting ureA and iceA genes. The correlation between iceA genotypes and gastritis was analyzed. Results Among 105 children, only 52 were possitive for H. pylori by the three methods. Among 52 children, there were 26 boys and 26 girls. Single iceA1 was detected in 78.84% （41/52） of children and single iceA2 was detected 1.92% （1/52）. Multiple strains infection of iceA1 and iceA2 was detected in 3. 84% （2/52） of children, iceA1 and iceA2 were not detected in 15.38% （8/52）. The comparison of the positive ratio of iceA1 in children infected with H. priori and that of other genotypes was significantly different（P 〈0. 01 ）. Among children infected with H. pylori iceA1,3 had mild chronic gastritis, 15 children had moderate chronic gastritis and 25 had severe chronic gastritis. Among children who were not infected with H. pylori iceA1,2 children had mild chronic gastritis, 4 children had moderate chronic gastritis, 3 children had severe chronic gastritis. The comparison of chronic gastritis in children infected with H. priori iceA1 and these not infected with H. pylori iceA1 was significantly different（P 〈0.05 ）. Conclusion iceA1 is the dominant genotype of H. pylori in children in Guangzhou in China. and iceA1 can resultoin more severe imflammation.