Vero细胞和流感病毒在无血清条件下的微载体培养

Serum-free Culture of Vero Cells and Influenza Virus on Microcarrier

目的探索Vero细胞和流感病毒在无血清条件下的微载体培养条件。方法在搅拌瓶中,选择合适的微载体,在无血清条件下培养Vero细胞和流感病毒。观察Vero细胞的生长状况,并检测流感病毒的血凝滴度。结果Vero细胞在Cytodex 3上生长最好,每个微载体上接种10个细胞为最佳接种浓度,以MOI=0.005和0.010 CCID50/细胞接种H1N1流感病毒后,在72h时,培养上清病毒血凝滴度达到最高。结论无血清条件下,Vero细胞在Cytodex 3上生长良好,流感病毒能在Vero细胞上成功培养。

Objective To explore the condition for serum-free culture of Veto cells and influenza virus on microcarrier. Methods Culture Vero ceils and influenza virus in serum-free medium by using microcarriers in stirring flask. Observe the growth and adsorption of Veto ceils and determine the heraagglutination titer of virus. Results Veto ceils grew well on Cytodex 3 microcarrier. The optimal seeding density of Veto cells was 10 cells per bead. The hemagglutination titer of virus reached the maximum 72 h after inoculateion at a MOI of 0. 005 or 0. 010 CCID50/cell. Conclusion In the absence of serum ,Veto cells grew well on Cytodex microcarrier, and influenza virus was successfully cultured in the cells.