COX-2抑制剂联合顺铂或X射线对A549肺腺癌细胞株的体外实验

The Depressive Effect of Detected on the Proliferation of A549 Human Lung Adenocarcinoma Cell Lines and Celecoxib Combined with Cisplatin or X-ray

目的观察环氧化酶-2(COX-2)抑制剂塞来昔布(Celecoxib)联合化疗药物顺铂(DDP)或联合X射线对A549人肺腺癌细胞增殖的影响及可能机制。方法应用MTS法分别检测Celecoxib与DDP联用对A549细胞增殖的影响及联合X射线对A549细胞存活分数(SF)的影响,流式细胞术检测细胞凋亡。结果在一定浓度范围内,Celecoxib和DDP均可抑制A549人肺腺癌细胞的生长,其抑制作用呈量-效关系。两者联用可增强对A549细胞生长的抑制作用,DDP浓度≥1mg/L时两者具有协同或相加作用。Celecoxib与X射线联用可显著降低细胞存活分数(SF),增加细胞凋亡率。结论Celecoxib与DDP联合可增强对A549人肺腺癌细胞的生长抑制效应;Celecoxib与X射线联合时,可增加A549人肺腺癌细胞的凋亡率,增强其对放射的敏感性。

Objective To observe the depressive effect of celecoxib combined with cisplatin or X ray on A549 human lung adenocarcinoma cells. Methods The depressive effect on the proliferation of A549 cells was detected by MTS technique when celecoxib was conbined with cisplatin, the effect on SF of A549 cells was detected by MTS technique when celecoxib was combined with X ray, the apoptosis of A549 cells was detected by flow cytometry. Results Within a certain concentration,celecoxib and cisplatin was able to inhibit the proliferation of A549 cells respectively, and dose-effect relationship was detected in the process. The combination of celecoxib （0. 35mg/L） and cisplatin could enhance the depressive effect, and synergistic effect or addition effect was detected in the combination when the concentration of cisplatin was more than 1mg/L. In addition, celecoxib/X ray（4Gy） combination significantly decreased the SF of A549 ceils and increased the apoptosis rate of A549 ceils. Conclusion Celecoxib/cisplatin combination could enhance the depressive effect on the proliferation of A549 human lung adenocarcinoma cells; Celecoxib/X ray could increase the apoptosis rate of A549 human lung adenocarcinoma cells, enhance the cells radio sensitivity.