补肾中药醇提活性成分对人牙周膜细胞生物学性状的影响

The effect of active components of Chinese medicine for tonifying kidney extracted by alcohol on the biological characteristics of cultured human periodontal ligment cells

目的:观察补肾中药醇提活性成分对体外培养人牙周膜细胞增殖和功能的影响。方法:应用组织块法和酶消化法体外培养人牙周膜细胞,以MTT法检测补肾中药活性成分对人牙周膜细胞的增殖率和中药活性成分对ALP活性的影响。结果:补肾中药醇提活性成分对人牙周膜细胞增殖能力和ALP活性有增强作用。结论:补肾中药醇提活性成分对体外培养人牙周膜细胞的增殖和功能有促进作用。

Objective：To investigate the effect of active components Chinese medicine for tonifying kidney extracted by alcohol on the proliferation and function of cultured human periodontal ligament cells.Methods ：The human periodontal ligament cells were cultured in vitro and the effect of active components of Chinese medicine for tonifying kidney extracted by alcohol on the proliferation ability and alkaline phosphatase （ALP）activity of hPDLCs were detected.Results：The active components extracted by alcohol strengthened the proliferation ability and ALP activity of cultured human periodontal ligament cells.Conclusions：The active components of Chinese medicine for tonifying kidney extracted by alcohol had great promotion on the proliferation ability and function of cultured human periodontal ligament cells.