胆色素结石模型胆汁中UCB、TBA、Ca^(2+)的动态实验研究

Dynamic Experimental Study on Unconjugated Bilirubin,Calcium Ion and Total Bile Acid Contents in Pigment Gallstone Model Bile

目的:在不影响动物胆汁酸池大小的情况下,在同一个体长期动态观察胆汁成分变化;研究胆色素钙结石形成的某些机制。方法:手术建立胆汁动态研究动物模型,待胆汁动态研究家兔模型稳定后,随机分为生理盐水组、胆道不全梗阻组、细菌感染组、内毒素组、胆道不全梗阻伴细菌感染组、胆道不全梗阻加内毒素组6组,动态观察各组胆汁中游离胆红素(unconjugated bilirubin,UCB)、总胆汁酸(total bile acids,TBA)、钙离子(calciumion,Ca2+)的变化。结果:生理盐水组胆汁中UCB、TBA、Ca2+无统计学意义改变(P>0.05),胆道不全梗阻伴细菌感染组、胆道不全梗阻加内毒素组胆汁中UCB、TBA、Ca2+变化明显,与生理盐水组比较具有非常显著性差异(P<0.01)。结论:改良的胆色素结石动物模型稳定可靠;胆道梗阻和感染是胆色素结石形成的重要原因。

Objective To establish correspondent animal model for the observation of dynamic changes of bile constituents, for further research on the pathogenesis of pigment stones Methods When the bile excretion was established, those rabbits were classified into six groups randomly, including saline group, incomplete obstruction group, infection group, lipopolysaccharide（LPS） group, infection and incomplete obstruction group and LPS and incomplete obstruction group. During the formation process of pigment stones, the above items of bile were also observed. Results No visiblechanges were found in the saline group. On the other hand, in the infection group, LPS and incomplete obstruction group, unconjngated bile（UCB）, total bile acid（TBA） and Ca^2＋ of bile showed visible changes. Conclusion This kind of bile modol is stable and reliable. Infection and obstruction of the biliary tract are causes of pigment stone formation.