结直肠癌中瘦素受体的表达及其在血管生成中的作用

The Expression of Leptin Receptor Ob-R and Its Angiogenic Effect in Colorectal Cancer

目的:研究瘦素受体(Ob-R)在结直肠腺癌组织中的表达;探讨Ob-R在肿瘤血管生成中的作用及其可能机制。方法:应用免疫组织化学方法检测35例结直肠腺癌患者癌组织和自身正常对照组织中Ob-R、CD105、核转录因子-κB(p65)的表达,以图像分析软件进行半定量测定。结果:癌症组中Ob-R和NF-κB(p65)的积分光密度以及CD105标记的微血管密度(MVD)均比对照组高。癌症组63.6%的标本中可见Ob-R血管内皮细胞染色阳性,其MVD较阴性者高(38.21±2.78比27.50±2.95,t=2.602,P<0.05);68.6%的癌症标本中可见NF-κB(p65)血管内皮细胞核表达阳性,其MVD较阴性者高(37.53±3.07比28.42±2.63,t=2.146,P<0.05)。Ob-R和NF-κB(p65)的表达部位一致,半定量分析显示二者正相关(r=0.705,P<0.05)。结论:结直肠腺癌组织中Ob-R表达增多对肿瘤血管生成起促进作用,并且该作用可能是通过NF-κB途径来实现的。

Aims： To investigate the expression of leptin receptor （Ob-R） in colorectal adenocareinoma tissue and to explore the effect of tumor angiogenesis and possible mechanism. Methods： Thirtyfive patients with colorectal adenocarcinoma, confirmed pathologically, were included in this study. Immumohistochemical staining was performed to detect the expression of the Ob-R, CD105, nuclear factor-kappa B （p65）（NF-κB （p65））in tumor tissues and their own normal controls. Image analysis software was used for semi-quantitative assessment. Results： The integrated optical density of the Ob-R and NF-κB （p65） and the microvessel density （MVD） marked by the CD105 in colorectal adenocarcinoma tissue were higher than in normal controls. The positive expression of Ob-R on vascular endothelial cells was seen in 63.6% of the cases of cancer group and the MVD in cases of the group was higher compared to the negative group （38.21±2.78 vs 27.50±2.95, t=2.602, P〈0.05）; the positive expression of NF-κB （p65） on the nucleus of vascular endothelial cells was seen in 68.6% of the cases of cancer group, and the MVD in the cases of the group was higher than the negative group （37.53±3.07 vs 28.42±2.63, t=2.146, P〈0.05）. The expression of the Ob-R and NF-κB （p65） on similar location was observed and, the semi-quantitative assessment showed that there was a significant association between the two （r=0.705, P〈0.05）. Conclusion： The study suggests that increased expression of Ob-R contributes to tumor angiogenesis in colorectal adenocarcinoma, probably through activation of the NF-κB pathways.