叶甲科三亚科十三种叶甲酯酶同工酶的研究(鞘翅目,叶甲总科)

STUDY ON ESTERASE ISOZYME OF THIRTEEN SPECIES OF CHRYSOMELIDAE

应用聚丙烯酰胺凝胶电泳方法分析研究了叶甲科3亚科13种昆虫的酯酶同工酶。结果显示,其聚类结果与传统分类结果基本相一致,说明以酯酶同工酶作为研究手段来进行叶甲类昆虫亚科以下阶元的分类是可行的,同时也说明了它们酯酶同工酶酶谱的差异和其分类地位是一致的;但跳甲亚科和叶甲亚科先聚为一类,再与萤叶甲亚科聚为一类,与前人的研究有差异,作者认为:酯酶同工酶的编码基因可能是快进化单位,在解决亚科以下阶元的系统关系时是很好的分子标记,而对于研究叶甲科、亚科间的系统关系,就不一定很合适。

The esterase isozyme （EST） of thirteen species of Chrysomelidae were examined by ways of vertical slab polyacrylamide gel electrophoresis in this study. The study shows the clustered result is accord with that of the traditional classification, but Chrysomelinae and Alficinae gather firstly, than they dose with Galerucinae. That result is different with that of Brian D. F. The author thinks the reasons have two points. The first, the genes coding EST of Galerucinae evolve slowly, and those genes may include many ancestry character informations, but the genes＇ evolution of Alficinae is quick. The second, the genes coding EST may be the rapid evolvement units.