膀胱癌细胞凋亡及p53基因表达的意义

Clinical Significance of Expression of p53 Protein and Apoptosis in Bladder Cancer

目的探讨膀胱癌细胞凋亡及p53基因表达的意义。方法采用免疫组织化学方法及TUNEL法,检测11例正常膀胱黏膜和83例膀胱移行细胞癌中p53基因的表达及细胞凋亡指数。结果83例膀胱移行细胞癌中p53阳性50.6%,凋亡指数(AI)=1.4335±0.3863。p53阳性表达与膀胱癌病理分级及临床分期呈正相关(γ=0.492,P<0.01;γ=0.341,P<0.01);凋亡指数(AI)与膀胱癌病理分级及临床分期呈正相关(γ=0.642,P=0.000<0.01;γ=0.455,P=0.000<0.01);AI与p53表达无相关性。AI、p53表达与患者5年生存率有关。结论临床联合运用临床分期及病理分级并检测p53可用于判断膀胱移行细胞癌的预后。

Objective To investigate the apoptosis and expression of p53 protein in bladder cancers, Methods Apoptosis Index（AI） was assessed with TUNEL technique ,and immunohistochemical stainings of p53 were performed in 83 cases of the bladder carcinomas and 11 normal bladder mneosa. Results The positive expression rate of p53 gene was 50.6% and AI = 1. 4335 ±0, 3863 in 83 cases of the bladder carcinomas. The expression level of p53 was positively correlated with the tumor pathological grade and clinical stage （γ=0,492,P 〈0.01 ;γ=0,341 ,P 〈0.01 ） ; The level of AI was positively correlated with pathological grade and clinical stage （ γ=0,642 ,P =0,000 〈0.01 ;γ=0.455 ,P =0.000 〈0.01 ） ; The level of AI was no correlated with p53. AI and the expression of p53 were related to 5-year survival rate, Conclusion Combined analysis of the tumor pathological grade, clinical stage and p53 genes expression is useful in providing prognostic information in bladder cancer.