10种金属离子与血清蛋白作用条件的研究

Study on the ReactionConditions of 10Metal Ions with Serum Protein

利用甲醇沉淀法和渗析法探讨了１０种金属离子与人血清蛋白的作用条件（浓度，温度及酸度），经用于毛细管电泳技术证明，金属离子－蛋白质的形成对消除毛细管堵塞有重要作用。应用本文研究所推荐的条件为，金属离子浓度１０～３０ｍｇ·Ｌ￣（－１），温度为４５℃，ｐＨ值为７．０。该１０种金属离子在此条件下与蛋白质作用的强弱顺序为Ｚｎ＞Ｃｕ＞Ｃｄ＞Ｃｏ＞Ｆｅ＞Ｃｒ＞Ｍｎ＞Ｃａ＞Ｎｉ＞Ｍｇ。

he reaction conditions of 10 metal ionswith human serum protein were studied bv using methanol.It was proved by the applicationof capillary zone electrophoresis technigue thatthe metal-protein can elminate the adsorptionof protein on the wall of capillary tube, Theproposed conditions for concentration of metalions’temperature and acidity are l0mg·L￣(-1)～30mg·L￣(-1) ,45℃ and pH 7 respectively. Theorder of reaction of metal ions with serum pro-tein under these conditions is as follows :Zn>Cu>Cd>Co>Fe>Cr>Mn>Ca>Ni>Mg.