马铃薯中配糖生物碱总量测定方法研究

Studies on the Determination Method for Total Glycoalkaloid in Potato

采用四氢呋喃∶水∶乙腈∶乙酸（５０∶３０∶２０∶１，Ｖ／Ｖ）混合液提取马铃薯中总配糖生物碱（ＴＧＡ）。离心后，上清液在酸性条件下超声波处理，氨水调节ｐＨ１０．５，沸水浴蒸干，用ｐＨ１０．５的氨水洗涤残留物，离心。７％磷酸溶解ＴＧＡ。室温下，加０．０３％聚甲醛磷酸溶液显色，３０ｍｉｎ后，于６２０ｎｍ波长处测定吸光度值，标准曲线法定量。对α－茄减的回收率为１００％～１０５％。

A procedure for deterrnining total gly-coalkaloids of potato is descrided in the pre-sent paper.Smashed potato was extracted withTFA-H_2O-CH_3CN-HAc[50∶30∶20∶1,(V/V)] solution and then centrifuged. The su-pernatant was subjected to ultrasonic treat-ment under acidic condition,and the pH wasadjusted to pH 10.5 with NH_3·H_2O. Waterbath was used to evaporate the solution,thenthe residue was washed twice with NH_3·H_2O(pH10.5)and dissolved in 7% H_3PO_4 solu-tion. Solution of 0. 03%paraformaldehyde wasused for color deveIopment at room tempera-ture for 30min,the absorbance was measuredat 620nm.Theresults indicated that therecov-erv of α-solanine was 100-105%.