聚吡咯葡萄糖氧化酶电极的生物电化学响应

Bioelectrochemical Responses of thePolypyrrole glucose Oxidase Electrode

采用分步聚合过程，制备了以聚吡咯膜为载体的葡萄糖氧化酶电极，探讨了其生物电化学响应特性，计算了酶催化反应的有关动力学参数。与溶解态酶相比，该电极表现出良好的生物电化学特性，而且酶蛋白对溶液温度的稳定性有显著提高。

glucose oxidase(GOD) electrode withpolypyrrole(PPy) membrane as the immobiliz-ing mediurn was prepared with a multiple-step process using the″ doping-undoping″property of PPy membrane,The bioelec-trochemical response characteristics were in-vestigated and the kinetic parameters of theenzyme-catalysed reaction were calculated ac-cording to the experimental results. In com-parison with the solubilized GOD ,the elec-trode exhibits good bioelectrochemical charac-teristics and the stabilitv of the enzyme pro-tein to the solution temperature is promotedsignificantly.