蝴蝶兰的组织培养和遗传转化体系的研究进展

Advances in tissue culture and genetic transformation system of Phalaenopsis

蝴蝶兰是观赏价值和经济价值很高的著名盆栽植物,用种子、茎尖、茎段、根尖、叶、花梗等外植体进行组织培养均获得了成功。不同外植体、不同培养基和不同激素种类及配比、不同浓度的6-BA和NAA组合对蝴蝶兰原球茎诱导率有显著影响。在兰花的转基因研究中,以基因枪法成功转化的报道最多,其次是农杆菌介导法,其它转化方法还有PEG法、电激法、花粉管通道法等。但仍需进一步探索和完善蝴蝶兰遗传转化体系,为以后目的外源基因的转入奠定基础,同时对于蝴蝶兰转基因技术的研究以及生物技术在兰花上的应用也具有重要的现实意义。

Phalaenopsis is a famous potted plant with high ornamental and economic value, the tissue culture of Phalaenopsis have been succeeded from the explants including seed, stem apex, stem section, root tip, leaf and flower peduncles, etc. In addition, the effects of different explants, medium, hormone type and combination, different concentration combination of 6-BA and NAA on the inducing rate of protocorm and differentiation of callus of Phalaenopsis are significant. Among the reports on the successful genetic transformation, most of them were about particle bombardment, followed by agrobacterium tumfefacients mediated procedure, and then other transformed methods included polyethylene glycol mediated transformation（PEG）, electroporation, pollentube pathway and so on. But, it is still necessary to explore and perfect the genetic transformation system of Phalaenopsis to provide the basis for the transformation of aiming alien gene in future, and it would have important practical significance to study the transgenic technology of Phalaenopsis and the application of biotechnology on Phalaenopsis.