广西肝癌组织中P^（53）蛋白和乙肝病毒免疫学标志物的检测

DETECTION OF P 53 MUTANT PROTEIN AND HEPATITIS B VIRUS IMMUNOMARKERS IN GUANGXI HUMAN HEPATOCELLULAR CARCINOMA TISSUE

用免疫组化法测定广西２９例人肝癌及其癌旁组织中的Ｐ５３蛋白、ＨＢｓＡｇ和ＨＢｘＡｇ。４４．８％肝癌病例为Ｐ５３蛋白阳性，其癌内及癌旁Ｐ５３蛋白的检出率分别为２７．６％和２８．６％。２１％的肝癌内及８５．７％的癌旁肝组织中可见到ＨＢｓＡｇ。３１％的癌内及７５％的非癌肝组织呈ＨＢｘＡｇ阳性。Ｐ５３蛋白阳性的１３例病例有１１例（８４．６％）可同时检测出ＨＢｓＡｇ和ＨＢｘＡｇ。结果显示：广西肝癌与Ｐ５３基因突变、乙型肝炎病毒（ＨＢＶ）感染关系密切。

P 53 mutant protein,HBsAg and HBxAg were detected with immunohistochemistry method in 29 cases of Guangxi HCC tissues and juxta tumor normal liver tissues 13 of 29 cases(44 8%) were positive for P 53 mutant protein HBsAg was detected in 6 of 29(21%) HCC cases and in 24 of 28(85 7%) surrounding liver tissues 9 of 29(31%) HCC cases and 21 of 28(75%) non tumour liver tissues were HBxAg positive Among 13 P 53 protein positive cases 11(84 6%) cases were associated with both HBsAg and HBxAg.The result shows that P 53 protein expression is high in Guangxi HCC patients,and HCC is closely related with P 53 mutation and HBV infection