小儿呼吸道感染病毒病原学研究

A STUDY ON THE VIRAL ETIOLOGY OF CHILDHOOD RESPIRATORY INFECTION

为了解近年来南宁地区小儿呼吸道感染病毒的流行趋势，采用桥联酶标法（Ａ－ＰＡＡＰ），对２６５例急性呼吸道感染患儿的鼻咽分泌物进行呼吸道６种常见病毒检测，同时采用ＥＬＩＳＡ法检测轮状病毒抗原。检测结果显示：阳性６１例，检出率为２３．０１％，其中合胞病毒３．０１％，腺病毒３．４％，流感Ａ５．６６％，流感Ｂ１．８９％，副流感Ⅰ、Ⅲ１．８９％，副流感Ⅱ３．０２％，轮状病毒４．１５％。６１例阳性患儿中，有１３例为混合感染（２１．１３％）。在各年龄组及各疾病组，病毒检出率无显著性差异，提示病毒仍是近年来本地区小儿急性呼吸道感染的一个重要病原。

In order to understand the epidemic trend of childhood respiratory tract viral infection in Nanning area these recent years,nasopharyngeal secretions(NPS) from 265 children with acute respiratory infection were tested for six common respiratory viral antigens by APAAP and for rotavirus antigen by ELISA There were 61 cases(23 01%) which gave postive antigen test,and the detectable rates of these viruses were as follows:influenza virus A(5 66%),rotavirus(4 15%),adenovirus(3 4%),parainfluenza Ⅱ(3 02%),respiratory syncytial virus (3 01%),influenza virus B (1 89%) and parainfluenza virus Ⅰ、Ⅲ(1 89%) There were 13 patients (21 13%) who had mixed viruses infection The differences in the detectable rates between different age groups and different respiratory diseases groups were not significant The results indicate that virus is still an important etiological factor in childhood acute respiratory infection in Nanning area these recent years