抑癌基因PTEN与iNOS在胶质瘤中表达及意义

Expression and Significance of the Tumor Suppressor Genes PTEN and iNOS in Glioma

目的探讨抑癌基因PTEN和诱导型一氧化氮合酶(iNOS)在胶质瘤组织中表达意义及两者与胶质瘤侵袭性的关系。方法采用LSAB免疫组织化学法检测56例胶质瘤中PTEN、iNOS蛋白的表达,并分析两者与胶质瘤病理级别和侵袭性的关系。结果PTEN在胶质瘤Ⅰ、Ⅱ、Ⅲ、Ⅳ级中阳性率分别为91．7％、75％、53．3％、15．4％,PTEN表达阳性率随病理分级升高而降低(P＜0．01)。而iNOS表达阳性率分别为50％、38．1％、73．3％、76．9％;iNOS表达水平与PTEN表达水平呈负相关(P＜0．05)。结论PTEN和iNOS的表达一定程度反映胶质瘤的恶性度和侵袭性强弱;PTEN缺失可引起iNOS表达增加,在胶质瘤发生、发展过程中发挥重要作用。

Objective To investigate the expression and significance of the tumor suppressor genes PTEN and iNOS in glioma,and analyze relationship between the expression and tumor invasion.Methods By using LSAB method,the expression of PTEN and iNOS in 56 cases of glioma tissues was detected,and their relationship histological grade and invasion ofglioma was analyzed.Results PTEN positive rate in glioma grades Ⅰ, Ⅱ, Ⅲ and IV were 91.7%,75%,53.3%, 15.4% respectively.The rate of PTEN expression was decreased with histopathological grading increase（P〈0.01）;While the positive rates expression of iNOS in glioma of grade Ⅰ, Ⅱ, Ⅲ and Ⅳ were 50%,38.1%,73.3%,76.9% respectively;An inverse correlation was found between PTEN and iNOS expression in glioma（P〈0.05）.Conclusion The expression of PTEN and iNOS may reflect the differentiation and invasive potential of glioma tissues to varying degrees.The deletion of PTEN can result in the overexpression ofiNOS,which may play an important role in the behavior of aggression in glioma.