差异表达基因Hepsin在前列腺癌中的表达及其意义

Expression and Significance of Differential Expression Gene Hepsin in the Prostate Cancer

目的探讨cDNA芯片筛选出的前列腺癌(PC)差异表达基因Hepsin在PC中的表达及其意义。方法应用RT-PCR检测Hepsin在正常前列腺组织、PC组织及PC细胞系中的表达,并进行半定量分析,同时采用免疫印迹法检测Hepsin的表达,采用免疫组织化学染色法检测40例PC标本、15例前列腺增生(BPH)标本和6例正常前列腺标本的Hepsin表达水平。结果在mRNA水平,Hepsin在PC中的表达水平高于正常前列腺组织(P=0.026),但在PC细胞系中的表达并不增高。在蛋白表达水平,正常前列腺组织未表达Hepsin,而PC及PC细胞系均表达Hepsin。PC和BPH标本免疫组化检测均为阳性,但两者间有非常高度显著性差异(P=0.000),而正常前列腺标本为阴性。结论Hepsin在PC中的表达增高,有可能作为早期诊断的标志以及基因治疗的靶基因。

Objective To evaluate the expression and significance of differential expression gene Hepsin in the prostate cancer （PC） screened by the cDNA microarray technique. Methods The techniques of semiquantitative RT-PCR and Western blotting were used to detect the mRNA and protein expression of Hepsin. Specimens of 40 cases of PC, 15 benign prostatic hyperplasia （BPH） and 6 normal prostate tissues were examined by the immunohistochemical stain. Results Hepsin was more expressed in PC tissue than normal prostate tissue （P =0. 026） and was confirmed by Western blot analysis. Immunohistochemical test confirmed this and demonstrated that Hepsin did not expressed in normal prostate but expressed in PC and BPH and there was a significant difference in Hepsin expression level between PC and BPH tissues （P =0. 000）. Conclusion Hepsin high expressed in PC may be a new molecular marker in early diagnosis of PC and a new target for gene therapy of PC.