E-cadherin,β-catenin,Cyclin D1在胃腺癌中的表达及临床意义

Expression of E-cadherin,β-catenin and Cyclin D1 in gastric adenocarcinoma and their clinical significances

目的:研究E-cadherin,β-catenin,Cyclin D1在胃腺癌组织中的表达,探讨三者的表达及临床病理意义．方法:采用免疫组织化学SP法检测73例胃腺癌组织及18例正常胃黏膜组织中E-cadherin,β-catenin,Cyclin D1蛋白的表达．结果:正常胃黏膜组织中E-cadherin和β-catenin均呈清晰的棕褐色染色在上皮细胞细胞膜．E-cadherin和β-catenin在胃癌细胞中出现细胞质和/或细胞核异常染色,其异常表达率分别为63．01%(46/73)和56．16%(41/73),且两者的异常表达与胃腺癌的分化程度、TNM分期、浸润深度及淋巴结转移相关．与患者的性别、年龄、肿瘤大小无关．胃腺癌中E-cadherin和β-catenin表达密切相关．Cyclin D1在胃腺癌组织中的阳性表达率为67．12%(49/73),明显高于其在正常胃黏膜组织中的表达(0%,0/18),且与胃腺癌的分化程度和淋巴结转移相关．E-cadherin和β-catenin在胃癌中的异常表达与Cyclin D1的过表达呈显著的正相关(r=0．249,r=0．376,P＜0．05)．结论:胃腺癌中存在E-cadherin和β-catenin基因的失活及蛋白表达下调．E-cadherin和β-catcnin的异常表达可能通过促使或激活Cyclin D1的过表达而参与胃癌的发生和发展．

AIM： To investigate the expression of E-cadherin, β-catenin and Cyclin D1 and their relationships and clinical pathological features in gastric adenocarcinoma. METHODS： Immunohistochemical SP method was used to determine the expression of E-cadherin, β-catenin and Cyclin D1 protein in 73 cases of gastric adenocarcinoma tissues and 18 cases of normal gastric mucosal tissues. RESULTS： Positive staining for E-cadherin and β-catenin was observed on the cellular membrane of epithelial cells in normal gastric mucosal tissues. The aberrant expression of E-cadherin and β-catenin were observed in thecytoplasm and/or cellular nucleus of gastric cancer cells. The aberrant expression rates of E-cadherin and β-catenin were 63.01% （46/73） and 56.16% （41/73） in gastric adenocarcinoma, respectively, which were associated with the differentiation degree, TNM stages, depth of infiltration and lymph node metastasis, but not with the gender, age and tumor size. There was a significant correlation between E-cadherin and β-catenin expression in gastric adenocarcinoma. The positive rate of Cyclin D1 expression was 67.12% （49/73） in gastric adenocarcinoma, notably higher than that in normal gastric tissues （0/18, P 〈 0.01）, and it was correlated with the differentiation degree and lymph node metastasis. The aberrant expression of E-cadherin and β-catenin were positively correlated with the high expression of Cyclin D1 gastric carcinoma （r = 0.249, 0.376, both P 〈 0.05）. CONCLUSION： Down-regulated expression of E-cadherin and β-catenin exist in gastric carcinoma, which may promote the pathogenesis and development of carcinoma by activation of Cyclin D1.