摘要
用便携式调制叶绿素荧光仪和光合仪研究了强光下不同供氮水平(12、4和0.4mmol.L-1)和不同CO2浓度下(700和390μl.L-1)丰香草莓叶片的荧光参数及净光合速率的变化.结果表明,CO2和氮素对草莓叶片光抑制有明显的互作效应.在富CO2下,12mmol.L-1供氮水平的草莓叶片净光合速率升高了62.7%,4和0.4mmol.L-1供氮水平则分别降低了7.4%和21.3%;12mmol.L-1供氮水平的Fm和Fv/Fm在强光胁迫时降辐减小,暗恢复时Fm和Fv/Fm恢复程度提高,而4和0.4mmol.L-1供氮水平却相反.表明氮素供应不足时草莓叶片在富CO2环境下光合作用出现适应性下调,光抑制增强.
By using PAM-2000 portable chlorophyll fluorometer and HCM-1000 photosynthesis measurement system, this paper measured the initial fluorescence ( Fo ), maximal photochemical efficiency of PSⅡ ( Fv/Fm) , maximal fluorescence ( Fm ), amount of inactive PS Ⅱ reaction centers ( Fi - Fo ) , proportion of QB-non-reducing PSⅡ reaction centers [ ( Fi - Fo ) / ( Fp - Fo ) ] , and net photosynthetic rate (Pn) of strawberry leaves under conditions of elevated CO2 (700 μl2L^-1) and ambient CO2(390 μl·L^-1 ) at three levels of nitrogen application ( 12, 4 and 0. 4 mmol·L^-1 ). The results showed that there was a significant joint effect between CO2 and N on the photoinhibition of strawberry leaves. Under elevated CO2 condition, the P. in treatment 12 mmol N·L^-1 increased by 62.7%, while that in treatments 4 and 0. 4 mmol N· L^-1 decreased by 7.4% and 21.3% , respectively. When exposed to high light and subsequently recovered in dark for 4 hours, the strawberry leaves in treatment 12 mmol N ·L^-1 showed less changes of Fm and Fv/Fm in elevated CO2 than in ambient CO2, while those in treatments 4 and 0. 4 mmol N ·L^-1 were in adverse, suggesting that for the strawberry leaves in elevated CO2, nitrogen deficiency could result in an acclimatized decrease of photosynthesis and an increase of photoinhibition.
出处
《应用生态学报》
CAS
CSCD
北大核心
2007年第1期87-93,共7页
Chinese Journal of Applied Ecology
基金
国家自然科学基金重点资助项目(39730340).
关键词
草莓
CO2加富
缺氮
叶绿素荧光
光抑制
strawberry
elevated CO2
nitrogen deficiency
chlorophyll fluorescence
photoinhibition