2,4,6-三氯苯酚诱导鲫鱼肝脏自由基的产生及其氧化应激

Free radicals in Carassius auratus liver:Their generation and oxidative stress induced by 2,4,6-trichlorophenol

采用电子顺磁共振的方法,研究了鲫鱼腹腔注射2,4,6-三氯苯酚(2,4,6-TCP)不同时间(4、8、12、24、72h)后其肝脏自由基强度的变化、氧化应激反应及其损伤机理.结果表明:2,4,6-TCP极显著促进了鲫鱼肝脏自由基的产生;鲫鱼肝脏内超氧化物歧化酶(SOD)与谷胱甘肽硫转移酶(GST)的活性受到显著诱导,过氧化氢酶(CAT)活性受到抑制,还原型谷胱甘肽(GSH)含量与对照组无明显差异,氧化型谷胱甘肽(GSSG)含量显著升高,丙二醛(MDA)含量极显著增加.

With electron paramagnetic resonance （EPR） technique, this paper studied the free radicals generation and oxidative stress in Carassius auratus liver after 4, 8, 12, 24 and 72 hours of 2, 4,6-trichlorophenol （2,4,6-TCP） intraperitoneal injection. The results showed that 2,4,6-TCP significantly promoted the generation of free radicals in C. auratus liver. The superoxide dismutase （SOD） and glutathione-S-transferase （GST） activities were induced significantly, while catalase （CAT） activity was inhibited, and glutathione （GSH） content had little difference with the control. A significant increase was observed in the contents of oxidized glutathione （GSSG） and malondialdehyde （MDA）.