摘要
应用RAPD技术研究了鳜塘水生生态系统中浮游生物群落α-多样性,分析了浮游生物群落DNA序列丰富度与水质和疾病发生之间的关系,探讨了池塘养殖密度和短周期、小密度、高投饵量养殖方式对浮游生物群落DNA序列丰富度及水质的影响.结果表明:1)鳜塘浮游生物群落DNA序列丰富度与水质综合指数呈显著负相关关系(P<0.01);2)池塘高密度养殖会造成浮游生物丰富度降低和水质综合指数升高;3)短周期、小密度、高投饵量的养殖模式对环境的损伤较大;4)浮游生物群落DNA指纹01矩阵和水质理化因子样品聚类分析表明,发病塘在水质理化因子和浮游生物群落上具有相似性,为鳜疾病预报模型的建立奠定了基础.
By using random amplified polymorphic DNA (RAPD) technique, this paper studied the a-diversity of plankton communities and its relationships with water quality and fish diseases in 7 Siniperca chuatsi ponds, as well as the effects of stocking density and a new culture model on the diversity and water quality. The results showed that there was a significant negative correlation between the DNA sequence diversity of plankton communities and water quality index, and high stocking density decreased the DNA sequence diversity and increased the water quality index. The new culture model with short culture period, low stocking density and high feeding stuff input had a greater damage on the water environment. Hierarchical cluster analysis indicated that there existed similarities in the DNA sequences of plankton communities and the physieoehemieal properties of water bodies in the ponds with fish diseases, which provided a possibility to predict the diseases occurrence in Siniperca chuatsi ponds.
出处
《应用生态学报》
CAS
CSCD
北大核心
2007年第1期163-168,共6页
Chinese Journal of Applied Ecology
基金
国家科技攻关计划项目(2004BA526B0501)
农业部农业结构调整重大技术专项(041105B)
广东省科技攻关资助项目(2002A20510).
关键词
鳜
RAPD
浮游生物群落多样性
理化因子
养殖密度
疾病
Siniperca chuatsi
RAPD
plankton community diversity
physicochemical factors
stocking density
disease.