siRNA敲除STAT3基因对结肠癌HCT116细胞侵袭的抑制

Inhibition of STAT3 siRNA on invasion of colon cancer cell line HCT116

目的:了解RNA干扰沉默STAT3基因对结肠癌细胞侵袭的可能作用。方法:根据STAT3基因特点,设计合成STAT3小干扰RNA(small interfering RNA,siRNA),并转染人结肠癌细胞系HCT116后,采用RT-PCR检测STAT3mRNA,采用软琼脂集落培养试验检测癌细胞锚着不依赖性增殖,采用Boyden小室模型试验检测癌细胞的侵袭能力。结果:STAT3 siRNA可有效抑制结肠癌细胞集落生长和侵袭能力,且与浓度相关。结论:STAT3 siRNA可抑制结肠癌细胞的侵袭。

Objective： To study the effects of STAT3 small interfering RNA （siRNA） on invasion of colon cancer cell line HCT116. Methods： After colon cancer HCT116 cell were transfected by STAT3 siRNA, we studied the expression of STAT3 mRNA using RT-PCR, anchorage-independent growth using clon formation in soft agar, and invasion using hoyden chamber mode. Results： STAT3 siRNA could knock-out the level of STAT3 mRNA. STAT3 siRNA can inhibit anchorage-independent growth and invasion ability in a dose-dependent manner. Condusion： STAT3 siRNA can inhibit anchorage-independent growth and invasion ability of colon cancer cell.