未修饰纳米SiO_2和表面修饰型纳米SiO_2对大鼠肺泡巨噬细胞的毒性研究

Study on cytotoxicity of nanosized silica and surface modified nanosized silica in rat alveolar macrophages

目的:比较未修饰纳米SiO2和表面修饰型纳米SiO2对大鼠肺泡巨噬细胞的毒性。方法:通过支气管肺泡灌洗分离雄性SD大鼠肺泡巨噬细胞。未修饰纳米SiO2(M-5型)及两种表面修饰型纳米SiO2(TS-610型,TS-720型)剂量分别为0,8,40,100,200μg/cm2,用MTT实验测定对细胞活性的影响,LDH实验测定对细胞膜的损伤,TBA法测定脂质过氧化作用的程度。结果:M-5型纳米SiO2在剂量大于100μg/cm2时,各指标与对照组相比有统计学意义(P<0.05),而两种表面修饰型纳米SiO2与对照组相比均无统计学意义。结论:未修饰纳米SiO2对大鼠肺泡巨噬细胞的毒性较强,颗粒表面被修饰后其毒性明显降低,表明颗粒表面反应性在毒性机制中发挥重要作用。

Objective： To compare the cytotoxicity of nanosized silica and surface modified nanosized silica in rat alveolar macrophages. Methods： Male SD rat alveolar macrophages isolated by bronchoalveolar lavage were exposed to nanosized silica （M-5） and surface modified nanosized silica （TS-610, TS-720） at doses of 0, 8, 40, 100, 200 μg/cm2. The cell activity was tested by MTr assay, injury to membrane by LDH assay and lipid peroxidation by TBA method. Results： For the three indices M-5 nanosized silica had significant difference from control when doses were higher than 100 μg/cm2 （ P 〈 0.05 ）, whereas surface modified nanosized silica had no significant difference from control at all doses. Condusion： M-5 nanosized silica was more toxic to alveolar macrophages. The apparent decrease of cytotoxicity after surface modification indicated the important role of surface reactivity in the cytotoxicity of nanosized silica.