交沙霉素的光度分析研究

Determination of Josamycin by Spectrophotometry

在一定条件下，稀H2SO4与交沙霉素（JSM）作用生成棕色配合物。该配合物的最大吸收波长为482nm，表观摩尔吸光系数ε为8．55×10^3L·mol^-1·cm^-1，交沙霉素在一定浓度范围内遵从比耳定律，线性范围为0～16．5mg／L。该法用于交沙霉素药剂中交沙素含量的测定，结果满意。

Under definite conditions, Diluted sulfuric acid react with josamycin （JSM） to form amber compounds with the maximum absorption wavelength of 482 nm and the apparent molar absorptivities （ε） of 8.55 × 10^3 L · mol^-1 · cm^-1. Based on this fact, a new spectrophotometric method for the determination of iosamycin has been developed. In the range of 0- 16.5 mg/L,the relationship between adsorption and concentration of josamysin obeys Beer＇s law. The method was applied to determine of josamycin with satisfactory results.