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PSA增强子启动子调控Smac基因表达载体的构建

Construction of expression vector carrying smac gene driven by prostate-specific antigen enhancer/promoter.
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摘要 目的构建携带Smac基因、人前列腺特异性抗原(PSA)增强子(PSAE)/PSA启动子(PSAP)调控的前列腺特异性真核表达载体。方法切除含Smac基因的真核表达载体pcDNA3.1-Smac内部的人巨细胞病毒/T7启动子序列,替换为在前列腺组织特异性表达的PSAE、PSAP调控序列。构建的质粒经双酶切凝胶电泳及测序鉴定。结果成功构建携带Smac基因人PSAE-PSAP调控的前列腺特异性真核表达载体pcDNA3-PSAE-PSAP-Smac质粒。结论新构建的载体为前列腺癌的靶向基因治疗奠定了基础。 Objective To construct the expression vector carrying Smac gene driven by human prostate-specific antigen enhancer/promoter（PSAE/PSAP）. Methods CMV and T7 promoters were deleted from eukaryotic expression vector pc DNA3. 1-Smac and replaced by human prostate-specific antigen enhancer/promoter. After restriction digestion and agarose electrophoresis of the recombinant vector, the new plasmid was confirmed by DNA sequencing. Results The eukaryotic expression vector pcDNA3-PSAE-PSAP-Smac was constructed successfully. Conclesion The new recombinant vector plays a basic role in prostate cancer-targeted gene therapy.
作者 邬喻 曾甫清 廖贵益 汪良
机构地区 华中科技大学同济医学院附属协和医院泌尿外科
出处 《华中医学杂志》 CAS 2007年第1期11-12,F0004,共3页 Central China Medical Journal
基金 国家自然科学基金资助课题(No.30271301)
关键词 前列腺肿瘤 前列腺特异性抗原 SMAC基因 启动子 增强子 Prostate neoplasms Prostate-specific antigen Smac gene Promoter Enhancer
分类号 R346 [医药卫生—基础医学]
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参考文献7

  • 1Verhagen AM,Ekert PG,Pakusch M et al.Identification of DIABLO,a mammalian protein that promotes apoptosis by binding to and antagonizing IAP proteins.Cell,2000,102(1):43
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二级参考文献10

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华中医学杂志
2007年 第1期

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