人结肠癌裸鼠移植瘤细胞动力学与雌孕激素受体研究

Investigation of DNA ploidy stattis ,cytokilnetics ,and female hormonalreceptors on nude mice with human colonic cancer

将人结肠癌异种移植于ＢＡＬＢ／ｃｎｕ／ｎｕ系无胸腺裸鼠并传５代。对原结肠癌及各代移植瘤进行病理学、组化、细胞动力学与电镜检查。应用流式细胞仪（ＦＣＭ）与生化葡聚糖包裹活性碳吸附法（ＤＣＣ）对各代移植瘤进行胞核ＤＮＡ倍体与雌孕激素受体（ＥＲ·ＰＲ）检测，并与原人结肠癌比较，结果显示：各代移植瘤均保持原人结肠癌相同的病理学特征、阳性雌孕激素受体表达及稳定的胞核ＤＮＡ倍体。就受体作用失常参与大肠癌发病机制进行讨论。

A colonic tubular adenocarcinoma from a 75-year-old woman washeterotransplanted to BALB/C nu/nu nude mice and five generations of trans-plantaton were conducted. The successful rate of transplantiaton ranged between 50%to l00%. Besides the studies of pathology, cytokinetics,and immunohistochemistry, wealso determined the DNA content using FCM and ER,PR by DCC method on cancersfrom each generation of nude mice xenograft and compared the ploidy status, DNAcontent, and distribution of ER and PR with original human colonic cancer. Ourpreclinical screening indicates that the xenograft cancer in each generation of nudemice maintained almost the same DNA content mostly aneuploids),positive results ofER and PR,and a stable cell-kinetics as the former human tumor. This investigationcan be beneficial to understand the imbalance of hormonal receptor as initiator andpronmotor for the development of some colorectal cancers which are considered ashormone-dependent tumors and provide the model to work out the genuine value ofendocrinotherapy with tamoxifen prospectively for colorectal cancers in the future.