NDPK／nm23在人肝癌中的表达及其与预后的关系

NDPK/nm23 expression and its correlation with prognosis in human hepatoce-llular carcinoma

目的：探讨肿瘤转移抑制基因ｎｍ２３的表达产物，二磷酸核苷激酶（ＮＤＰＫ）在人肝癌组织中的表达及其与预后的关系。材料和方法：材料取自河南省肿瘤医院１９８７～１９９３年肝癌切除的部分手术标本，共５４例。抗ＮＤＰＫ小鼠单克隆抗体和免疫组化试剂盒购自Ｄａｋｏ公司。研究方法按ＬＳＡＢ法免疫组织化学操作技术常规进行。结果：５４例肝癌组织中，ｎｍ２３表达阳性率为５９．３％（３２／５４），患者年龄、肿瘤的大小、肿瘤的大体分型、肿瘤分期、分级等在ｎｍ２３表达的阳性和阴性组间的分布差异无显著性性（Ｐ＞０．０５）。生存期分析表明，ｎｍ２３表达阴性组的生存期短于阳性组。结论：ｎｍ２３表达阴性与肝癌预后不良有关，但多因素分析显示，ｎｍ２３不能作为判断肝癌预后的独立指标。

Purpose:To explore the expression of nucleoside diphosphate kinase-(NDPK),the metastatic suppressor gene and correlation between the nm23 protein andthe prognosis in patient with hepatocellular carcinoma. Materials and methord: 54hepatic specimens came from Henan Tumor Hospital between l987～1993.the mousemonoclonal antibody was brought from Dako Co. 54 specimens were studiedimmunohistochemically. ResuIts:When tested, positive expression of nm23 was59.3%(32/5).there was no significant correlation between it and the negative groups(P>0.05).Analysis of survival period showed patients in nm23 negative group wasshorter as compared with those with nm23 positive groups. Conclusion :Patients withnegative nm23 had poor prognosis,but multifactorial analysis showed nm23 proteinmight not be the single independent prognostic indicator in hepatocellular carcinoma.