高浓度臭氧水稳定性及杀菌效果的试验观察

EXPERIMENTAL OBSERVATION ON STABILITY AND GERMICIDAL EFFICACY OF HIGH-CONCENTRATION OZONE WATER

目的观察高浓度臭氧水杀菌效果及其在不同条件下的稳定性。方法采用定量杀菌试验方法和化学测定方法在实验室进行了观察。结果用臭氧水消毒机生产制备的臭氧水浓度为13．23—44．73mg／L。在5℃-25℃条件下，臭氧水的半衰期在150．7～12．5min范围内依次递减。用含量为13．73mg／L的臭氧水，对悬液中金黄色葡萄球菌、大肠杆菌、白色念珠菌作用5min，平均杀火率为分别99．99％、99．95％、99．90％；对枯草杆菌黑色变种芽孢作用20min，平均杀灭率达到99．99％。用16．58mg／L的臭氧水，对载体上金黄色葡萄球菌、大肠杆菌、白色念珠菌作用20min，平均杀灭率分别为99．92％、99．95％、98．75％；对枯草杆菌黑色变种芽孢作用60min，平均杀灭率为99．95％。结论用臭氧水消毒机可以制备出浓度高达44．73mg／L的臭氧水；这种臭氧水在常温下分解速度比较快；以13．73mg／L和16．58mg／L臭氧水分别对悬液内和载体上细菌繁殖体、真菌和细菌芽孢均有较强的杀灭效果。

Objective To observe germicidal efficacy of high - concentration ozone water and,its stability under different conditions. Method Quantitative germicidal test method and chemical measuring method were used to carry out laboratory observation. Results The concentration of ozone water produced by ozone water disinfector was 13.23 - 44.73 mg/L. Under 5℃- 25℃ conditions, the half life of ozone water decreased progressively in the range of 150.7 - 12.5 min. The average killing rates of Staphylococcus aureus, Escherichia coli and Candida albicaus in suspension exposed to the ozone water containing ozone 13.73 mg/L for 5 min were 99.99%, 99.95% and 99.90% respectively and that ofBaciUus subtilis var. niger spores exposed for 20 min was 99.99%. The average killing rates of Staphylococcus aureus, Escherichia coli and Candida albicaus on carriers exposed to 16.58 mg/L ozone water for 20 rain were 99.92%, 99.95 % and 98.75% respectively and that of Bacillus subtilis var. niger spores exposed for 60 min was 99.95%. Conclusion The ozone water disinfector can produce the ozone water with concentration as high as 44.73% mg/L. This kind of ozone water decomposes rapidly at room temperature. The ozone water containing ozone 13.73 mg/L or 16.58 mg/L has high efficacy in killing vegetative forms of bacteria, fungi and bacterial spores in suspension or on carriers.