蛋黄降压肽酶解液脱色工艺的优化

采用单因素和正交实验法,用粉末活性炭对含蛋黄降压肽的酶解液进行脱色处理,考察了活性炭用量、pH、脱色温度和吸附时间等因素对脱色率、肽损失率和溶液活性损失率的影响。结果表明,粉末活性炭用量3%,pH4.5,脱色温度40℃,吸附时间30min的条件下,蛋黄降压肽酶解液脱色效果明显,肽损失率(LP)可控制在20%以下,脱色率(DE)可达到86.3%,而酶解液对血管紧张素酶(ACE)的抑制率(IP)为77.89%,活性损失率为6.39%。

Activated carbon was applied to decolour egg yolks hydrolysate including ACEI by single factor and orthogonal experiments. The factors including quantity of activated carbon, pH, and temperature and adsorption time were investigated in terms of decolouring efficiency, egg yolks peptide loss and bioactivity. The results showed that optimum decoloration effect was obtained from the following conditions：quantity of activated carbon 3%, pH value 4.5, temperature 40℃, and adsorption time 30 minutes. Then decolouring efficiency, egg yolks peptide loss and inhibition percentage were 86.3%, below 20% and 77.89%. and bioactivity loss was 6.39%.