春花胡枝子黄酮类化合物的提取及清除羟自由基作用的研究

Study on Flavonoids Extraction and Function of Scavenging Hydroxy Free Radical from Lespeda.dunnii.

采用不同的提取剂处理春花胡枝子叶,所得提取物与芦丁在紫外、可见光区的光谱对照及黄酮类化合物的特征显色反应,证明了春花胡枝子含有黄酮类化合物。通过单因素实验及正交试验对春花胡枝子黄酮提取的工艺条件进行了探讨。结果表明,春花胡枝子黄酮提取的最佳工艺条件是温度75℃、乙醇浓度75%、料液比1:30、提取时间为3.5h,此条件下黄酮的提取率为89.5%。此外,还对春花胡枝子提取物的抗氧化活性进行了初步研究。

The leaves of Lespeda.dunnii were dealt with different extraction reagents. The flavonoids extracted were proved to exsist in Lespeda.dunnii. by the characteristic coloration reactions of the flavaonoids in comparison to UV and visible spectrum shown between the exWacted substance and turin.The technological conditions were approached by single factor and orthogonal test for the extraction of the flavonoids from Lespeda.dunnii.The results showed that the optimum technological conditions are： temperature at 75℃, the extraction reagent of 75% ethanol, material to exWaction reagent 1：30 and the exWaction time 3.5h. The flavonoids ratio yield of exWaction reaches 89.46%. In addition, The anti-oxidant capacity of the flavonoids from Lespeda.dunnii. was preliminarily studied, as well.