摘要
经水提、醇沉、Sevag法脱蛋白的藤茶多糖(AGP),通过DEAE-SephadexA-25和SephadexG-200柱层析,得到多糖AGP-3和AGP-4。HPLC和SephadexG-200柱层析鉴定AGP-3和AGP-4为分子量相对均一的蛋白多糖,硫酸-咔唑反应、考马斯亮蓝G-250反应均呈阳性。
Polysaccharide from Ampelopsis grossedemata （AGP） was obtained after being extracted with hot water, precipitated with alcohol and removed flee protein with Sevag method. AGP was isolated and purified by DEAE-Sephadex A-25 and Sephadex G-200 column chromatography, two pure polysaccharides （AGP-3 and AGP-4） were gained. The results of HPLC and Sephadex G-200 column chromatography illustrated that both AGP-3 and AGP-4 were protein-bond polysaccharids with comparatively homogenous molecular weights. Also, the two polysaccharids were colored by coomassie brilliant blue G-250 and carbazole reaction.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2007年第1期151-154,共4页
Food Science
基金
湖北省自然科学基金项目(2006)
湖北省"十五"重点科技攻关项目(2001AA204A03)
关键词
藤茶
多糖
分离
纯化
性质
Ampelopsis grossedentata
polysaccharide
isolation
purification
characterization
