摘要
应用N+离子注入诱变技术对环糊精葡萄糖基转移酶产生菌进行诱变选育高产菌株。筛选到产酶水平是出发菌株1.6倍的菌株B.sp.HA-1,该菌株于5m3生产罐上发酵24h产酶能力达到6800IU/ml。酶转化25%木薯淀粉生成β-环糊精,12h转化率达到85%。该菌株产酶具有良好的传代稳定性,F2～20代产酶水平是F1代的98.11%～102.66%。
With N^＋ ion implantation, a high-yield cyclodextrin glucanotransferase processing strain Bacillus sp.HA-1 was selected, with CGTase activity level 1.6 times of that of original strain. Its CGTase activity reached 6800IU/ml when cultivated for 24 hours in 5m^3 fermentor. Using the CGTase to transform 25% tapioca starch into 13 -CD, the convertion rate in 12 hours is 85%. The CGTase activitise of F2-F20 are ranged from 98.11% to 102.66% as compared with F1, which proves that the genetic quality ofB. sp. HA-1 strain is stable.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2007年第1期161-164,共4页
Food Science
基金
河南省科技攻关项目(001160201)
