玉米萌发过程中蛋白酶的活力和蛋白质含量的研究

Study on Proteinse Activity and Protein Content in Corn during Germination

本文研究了玉米萌发过程中各种因素对其中所含蛋白酶活力的影响,并研究了获得最高蛋白酶活力度最佳萌发条件,通过SDS-PAGE凝胶电泳确定了玉米萌发后可溶性蛋白的分子量。结果表明,水分含量、温度、光照以及赤霉素等因素对玉米蛋白酶活力均有影响,在水分35%、温度30℃、赤霉素30mg/L和自然光照条件下萌发,玉米的蛋白酶活力最高;在萌发6d后,SDS-PAGE图谱中出现了20kD以下的两条明显谱带,b带分子量测定结果为19.2kD,c带分子量测定结果为18.2kD。

Factors influencing the activity of proteinase in corn during germination were studied, and the molecular weight of soluble protein after corn germinating was determined by SDS-PAGE. The results showed that the proteinase activity is affected by moisture content, temperature, light and gibberellin acid （GA）. The maximum activity of proteinase was obtained on the following conditions, moisture content 35%, temperature 30℃, gibberellin acid 30mg/L, natural light; after germination for 6 days, there were two obvious protein bands with the molecular weight lower than 20kD, the coleculare weight of band band c was 19.2kD and 18.2kD, respectively.