HPLC法测定龙眼肉中的几种核苷类物质

Quantitative Determination of Several Kinds of Nucleotides in Dimocarpus longan Lour by HPLC

目的:建立HPLC法测定龙眼肉中腺苷、尿苷和腺嘌呤的含量。方法:采用NucleosilC18反相柱(250mm×4mm),以0.01mol/L的KH2PO4:乙腈=92:8为流动相,流速为1ml/min,检测波长为254nm,柱温40℃,用外标法,测定了龙眼肉中腺苷、尿苷和腺嘌呤的含量。结果与结论:分析中选取腺苷的线性范围0.1～20.0μg/ml,相关系数r=0.9999,回收率94.59%,RSD3.10%;尿苷的线性范围0.05～10.0μg/ml,相关系数r=1,回收率97.49%,RSD4.50%;腺嘌呤线性范围0.05～10.0μg/ml,相关系数r=1,回收率103.11%,RSD1.90%。该方法简便快速,线性关系良好。

Objective： To determine the content of adenosine, uridine and adenine in Dimocarpus longan Lour. Methods： The HPLC method was used to determine respectively the content of adenosine, uridine and adenine in Dimocarpus longan Lour. The analysis was carried out on Nucleosil C18 column（2.50mm×4mm）. The mobile phase was 0.01mol/L KH2PO4-CH3CN （92：8, V/V）, flow-rate 1.0ml/min; wavelength 254nm and temperature 40℃. Results and conclusion： The method is simple and has a good linear relationship. The linear range of adenosine is 0.1-20.0μg/ml and gives a correlation of 0.9999.The recovery of adenosine is 94.59% and RSD 3.10%. The linear range of uridine is 0.05-10.0μg/ml and gives a correlation 1. The recovery of uridine is 97.49% and RSD 4.50%. The linear range of adenine is 0.05-10.0μg/ml and gives a correlation of 1. The recovery of adenine is 103.11% and RSD 1.90%. The method is easy to determine the content of several kinds of nucleotide in Dimocarpus lon gan by HPLC.