毛细管气相色谱法分析猕猴桃中的有机酸

Analysis of Organic Acids in Actinidia chinensis by Capillary Gas Chromatography

本文采用气相色谱定量分析了猕猴桃中有机酸含量。采用10%的硫酸甲醇对样品进行衍生化,用二氯甲烷萃取,最后进行气相色谱分析。结果表明,该方法的回收率高(93.8%～102.3%),相关性好(0.9991～0.9998),重复性好(RSD<5%)。猕猴桃中的有机酸主要是苹果酸、柠檬酸、棕榈酸、油酸和亚油酸等。

A method was developed for the quantitative analysis of organic acids in Actinidia chinensis by GC. The sample was ethefified in 10% H2SO4-CH3OH, followed by extraction with CH2Cl2, and determined by GC. The results showed that method has high recovery（93.8%-102.3%）, good correlation（0.9991 -0.9998） and good reproducibility（RSD〈5%）. The main organic acids in Actinidia chinensis are：malic acid, citric acid, palmitic acid, oleic acid and linoleic acid.