单核细胞趋化蛋白-1和核因子-κB与巨噬细胞浸润及动脉粥样硬化斑块形成的关系

Relations between monocyte chemotactic protein-1,nuclear factor-kappa B and macrophage infiltration,and coronary artery plaque formation

[目的]研究单核细胞趋化蛋白-1和核因子-κB与巨噬细胞浸润、冠状动脉粥样硬化宽块形成及冠状动脉进行性狭窄的关系。[方法]从39例尸检标本中获得39个冠状动脉左前降支粥样硬化的标本。应用弹力纤维染色及电脑软件分析系统，测量其狭窄程度，并根据其狭窄程度将其分为3组（A组：〈50％；B组：50％～75％；C组：〉75％）。B组与C组中又根据粥样斑块占内膜面积的百分比分别以20％和30％为界，分为两个亚组，即B1（〈20％）、a2（≥20％）和C1（〈30％）、C2（≥30％）。用免疫组织化学的方法来检测巨噬细胞（CD68），单核细胞趋化蛋白-1和核因子-κB在冠状动脉宽块中的表达情况。[结果]①B组与C组的中膜巨噬细胞浸润的个数（6．38±7．68,6．09±4．95）明显高于A组（0．78±0．67），P〈0．05。B2和C2的宽块面积占内膜面积的百分比（42．94±6．54，67．20±15．63）和巨噬细胞数（189．17±78．92，195±71．93）明显高于B1（16．74±3．18，5．20±27．52）和C1（16．84±4．89，110±13．60），P〈0．05。②MCP-1和NF—κB的阳性表达出现在粥样宽块处的内皮细胞、巨噬细胞及平滑肌细胞，尤以在泡沫细胞处更加明显；而且两者之间存在显著正相关（r=0．632，P〈0．001）。[结论]动脉局部核因子-κB和MCP-1的过度表达可能参与并调节局部炎症反应及巨噬细胞的浸润，而且与冠状动脉粥样硬化斑块形成及管腔进行性狭窄关系密切。

[Objective ] To investigate the correlation between monocyte chemotactic protein - 1, nuclear factor - kappa B and the macrophage infiltration into intima, then and the formation of coronary atherosclerotic plaque, the last and the progressive stenosis of coronary arteries. [ Methods ] The samples, thirty - nine left anterior descending coronary arteries, were gotten from thirty - nine autopsied cases. The stenosis degree was measured by elastic fibers straining and software analysis system. Then according to the degree, they were divided into3 groups （A： 〈 50% ; B： 50% -75% ; C： 〉75% ）. Based on the percentage of the plaque in intima, both B and C were divided into two sub-groups （B1 〈20% ,B2≥20% ;C1 〈30% ,C2≥30% ）. The expression of macrophages（CD68）, MCP - 1 and NF - κB was examined by immunohistecbemistry. [ Results] ①The macrophages counting in media of B and C（6.38±7.68, 6.09 ±4.95 ） were much more than that of A （ 0.78 ± 0.67 ）, P 〈 0. 05. The plaque area and macrophages counting of B2 and C2 （42.94 ±6.54,189.17 ±78.92; 67.20 ±15.63,195 ±71.93）were obviously larger than B1 and C1（16.74 ±3.18,5.20 ±27.52; 16.84 ± 4.89 , 110 ± 13.60 ）, P 〈 0.05. ②MCP - 1 and NF - κB expressed in endothelium cells, macrophages and smooth muscle cells, especially in foam cells. And there was positive relationship between them （r = 0. 632,P 〈 0.001 ）. [ Conclusion ] The excessive expression of MCP - 1 and NF - κB in coronary atherroselerosis probably regulated the local inflammation and the macrophage infiltration, and was closely related to the formation of coronary atherosclerotic plaques and the progressive stenosis of coronary arteries.