全身应用激素对鼻息肉组织中Eotaxin表达的影响

Effects of systemic corticosteroids on expression of Eotaxin in nasal polyps

目的:观察全身应用糖皮质激素对鼻息肉组织中Eotaxin表达的影响。方法:采用免疫组织化学SP法,检测在激素治疗前后Eotaxin在鼻息肉组织中的表达,运用HPIAS-2000高分辨率图像分析系统分别对激素治疗前后鼻息肉组织的黏膜上皮细胞、血管内皮细胞、炎性细胞进行吸收度测定。结果:糖皮质激素治疗前,Eotaxin阳性表达主要分布在鼻息肉组织的黏膜上皮细胞、炎性细胞及血管内皮细胞的细胞质中;激素治疗后,Eotaxin在鼻息肉组织黏膜上皮细胞、炎性细胞、血管内皮细胞中表达显著降低,且均差异有统计学意义(均P<0.05)。结论:糖皮质激素抑制鼻息肉组织黏膜上皮细胞,炎性细胞,血管内皮细胞中Eotaxin蛋白的表达,减少嗜酸粒细胞募集和活化,从而减轻鼻息肉的嗜酸粒细胞性炎症反应程度,可能是其治疗鼻息肉的作用机制之一。

Objective：To observe the effects of systemic corticosteroids on the expression of Eotaxin in nasal polyps. Method：The SP immunohistochemical methods were applied to detecte the expression of Eotaxin in nasal polyps before and after corticosteroids therapy. The optical density of positive cells in mucosal epithelia, vascular endothelia,inflammatory cells was measured by using H PIAS 2000 image conduct system. Result： The expression of Eotaxin was positive in the cytoplasm of the mucosal epithelia, the inflammatory cells and the vascular endothelia in nasal polyps. The expression of Eotaxin was significantly reduced in the mucosal epithelia , the inflammatory cells and the vascular endothelia of nasal polyps after treated with corticosteroids compared with pre-treated. Con- clusion： Inhibiting the expression of Eotaxin in mucosal epithelia,inflammatory cells and vascular endothelia of na- sal polyps, reducing recruitment and activation of eosinophile and relieving eosinophile inflammatory reaction in nasal polyps may be one of mechanisms of systemic corticosteroids therapy on the nasal polyps.