赖氨酸阿司匹林对庆大霉素耳毒性的保护作用

Protective effects of lysine aspirin against gentamicin ototoxicity

目的探讨庆大霉素与赖氨酸阿司匹林混合用药对庆大霉素耳毒性的保护作用。方法健康♂豚鼠30只,随机分为3组:庆大霉素(GM)组、庆大霉素联合赖氨酸阿司匹林(GM+LAP)组和对照组。GM组:12mg·kg-1,sc,qd;GM+LAP组:GM 12 mg·kg-1+LAP 135 mg·kg-1,sc,qd。对照组:不给予任何药物。采用听性脑干反应(ABR)和耳蜗铺片观察用药前后听阈及耳蜗毛细胞形态学改变。结果GM组1、8 kHz ABR4 wk平均阈移与GM+LAP组间差异有统计学意义(P<0．05)。耳蜗铺片结果显示,GM组底圈毛细胞有损伤,与正常组比较排列不整齐,毛细胞损伤向上逐渐减轻,与听阈升高的程度相平行;而GM+LAP组的毛细胞排列整齐,无损伤。结论赖氨酸阿司匹林对庆大霉素耳毒性有保护作用。

AIM To study protective effects of lysine aspirin against gentamicin ototoxicity in guinea pig. METH-ODS Thirty healthy male pigmented guinea pigs were randomly divided into 3 groups （each 10）. The usual clinical doses were used both in gentamiein（GM） group and GM ＋ lysine aspirin（LAP） group in order to observe the safety and effect of the mixed drug. GM group： 12mg·kg^-1, sc,qd; GM ＋ LAP group： GM 12mg·kg^-1＋LAP 135 mg·kg^-1, sc,qd. RESULTS GM group developed a progressive threshold shift had difference. Injury in high frequency was significantly more severe than that in low frequency （P 〈 0.05）. Hearing loss of high frequency was heavier than that of low frequency. Thresholds of control group and LAP group maintained steadily. Morphological results were comistent with functional changes. The worst damages occurred in the basal circles. Damages in upper circles were lighter. CONCLUSION These results suggest that lysine aspirin may be a promising therapeutic agent to gentamiein ototoxieity.