胃缺血和再灌注时胃表面激光多谱勒血流图像的显示

Display of Laser Doppler Perfusion Imaging(LDPI) for Stomach Blood Flow in Ischemia-Reperfusion

目的:连续检测和显示胃脏缺血和再灌注图像,研究胃脏血液供应血管的分布及激光多谱勒血流成像新技术在内脏血循环检测中的价值。方法:对大鼠胃右动脉进行结扎和松解,用激光多谱勒血流灌注成像仪(LDPI)对该过程中的胃脏整体表面循环予以图像显示,分析结扎和松解时胃血流的改变。结果:(1)胃脏表面的血流灌注图像显像清晰,呈现出从胃小弯向胃大弯放射状血流分布的扇形特征;(2)胃右动脉结扎后,激光多谱勒血流图上胃表面血流灌注下降非常显著,一直持续到松解结扎;(3)松解结扎后,血流即刻骤升,前10min内血流灌注量可超过结扎前水平,随后逐渐平稳。结论:结扎胃右动脉可以形成胃脏缺血和再灌注模型,LDPI能将胃脏的血循环状态以图像的形式大范围显示。

Objective To continuously display the images of stomach blood flow in ischemia-reperfusion, in order to research supplier for stomach blood flow, and to evaluate the application value of the Laser Doppler perfusion imaging （LDPI）, the new medicinal image technique, on the measure of entrails blood circulation. Methods During the right gastricartery was clamped, the image of blood flow perfusion of stomach was displayed in the rat by LDPI, and change of blood circulation was analyzed. Results （ 1 ） In the nature state the blood flow perfusion images of the stomach are clear. LDPI image of stomach shows in flabellate distribution of blood vessel from lesser curvature to greater curvature of stomach. （2）After clamped the right gastricartery, the blood flow perfusion is going down markedly on the LDPI images, and last out loosened artery. （3）After loosened artery, the blood flow flare up to increase, and is paranormal. During 10min to 30min loosened artery the blood flow come back to normal. Conclusions The model of ischemia-reperfusion is came into being by clamping the right gastricartery, the blood flow perfusion of the entrails could be displayed in imaging by LDPI.