血液保存条件与SE-9000分析仪检测外周造血干细胞的稳定性

Effects of preservation condition on the stability of hematopoietic stem cells in peripheral blood examined by blood analyzer SE-9000

选择40例第四军医大学西京医院1998/2005期间住院患者,作为干细胞移植受者。其中脐带血干细胞移植受者20例,男11例,女9例;骨髓干细胞移植受者20例,男13例,女7例。干细胞移植后第25天,抽取干细胞移植受者外周血液40mL,注入含60mgEDTA-K2的三角烧瓶中充分混匀,即刻(0h)在血液分析仪上进行检测,后将血液样本分装在27支试管中,并分成3组,每组9支,各组分别放置于4℃、20℃、30℃条件下保存。在取血后的1,2,4,6,8,12,24,48,72h从各组(4℃、20℃、30℃)中分别取出1支在血液分析仪上对其造血干细胞数量及其所占百分率进行检测,并记录结果。结果显示,4℃条件下保存12h造血干细胞的数量和百分率无明显变化,20℃条件下保存8h造血干细胞的数量和百分率无明显变化,30℃条件下保存6h造血干细胞的数量和百分率无明显变化,8h开始结果逐渐降低。

Forty inpatients ware selected from Xijing Hospital Affiliated to Fourth Military Medical University of Chinese PLA between 1998 and 2005 as the recipients of stem call transplantation, including 20 cord blood （CB） stem call recipients （11 males and 9 females）, 20 bone marrow stem cell recipients （13 males and 7 females）. On the 25^th day of stem call transplantation, 40 mL of peripheral blood was obtained from the recipients and injected into a conical flask containing 60 mgEDTA-K2 to mix together for immediate detection with blood analyzer. The blood sample was input into 27 test tubes respectively and divided into 3 groups with 9 tubes in each group, which were then stored at 4 ℃, 20 ℃ and 30 ℃ respectively, One tube was obtained from each group respectively on the 1^st, 2^rd, 4^th, 6^th, 8^th, 12^th, 24^th, 48^th and 72^th hours after blood drawing to detect the number of hematopoietic stem call （HSC） and the percentage with blood analyzer, and the results was recorded. The results showed that there was no significant difference in number and percentage of HSC after being stored at 4 ℃ for 12 hours as wall as being stored at.12 ℃ for 8 hours and being stored at 30 ℃ for 6 hours, while they gradually decreased from the 8^th hour.