养心颗粒对急性心肌梗死犬血管内皮细胞及心肌损伤的保护作用

Protective effect of Yangxin granules on endothelial cells and myocardium in mongrel dogs with myocardial infarction

目的观察养心颗粒（YXG）对急性，心肌梗死犬血管内皮细胞及心肌损伤的保护作用。方法采用结扎冠脉前降支的方法，复制急性，心肌梗死模型。分别测定冠脉结扎前以及药后不同时间点血浆超氧化物歧化酶（SOD）、丙二醛（MDA）、内皮素（ET）、一氧化氮（NO）、P-选择素及肌酸磷酸激酶同工酶（CK-MB）的变化。采用N-BT染色法测定心肌梗死面积。结果YXG治疗组与模型组比较，血浆CK-MB、MDA、ET、P-选择素降低（P〈0．05—0．01），而血浆SOD和NO升高（P〈0．05-0．01），梗死面积明显缩小（P〈0．01）。结论YXG对急性，心肌梗死犬心肌及血管内皮细胞的损伤具有保护作用，其作用机制可能与抗氧化、改善血管内皮细胞的分泌功能及抑制血小板活化有关。

Purpose To study the protective effect of Yangxin granules on vascular endothelial cells and myocardial injury in mongreldogs with myocardial infarction. Methods A total of 36 mongrel dogs were randomly divided into six groups,namely sham-operated group,myocardial infarction（MI） group,diltiazem group, and Yangxin granules 5,15,30 mg/kg groups.The experimental animals were handled by ligating the left coronary artery at the anterior descending branch, and a blood sample was withdrawn to measure the level of plasma ereatine kinase isoenzyme （CK-MB）, the activity of superoxide dismutase （SOD）, the content of malondialdehyde （MDA） and nitric oxide（NO） and the concentration of plasma endothelin（ET） and P-selectin. Infarction size was also determined. Results Infarction size was significantly less in Yangxin granules 15,30 mg/kg groups and diltiazem group compared with MI group（ P 〈 0.01 ）. Plasma CK-MB activity（ P 〈 0.05 - 0.01 ） was significantly reduced in Yangxin granules 15,30 mg/kg groups vs MI group.Plasma MDA,a product of lipid peroxidation,was significantly decreased（ P 〈 0.05） in Yangxin granules 15,30 mg/kg groups vs MI group,cansistent with the increased plasma SOD activity（ P 〈 0.01 ）. The level of plasma NO（ P 〈 0.01） was comparably increased in Yangxin granules treated groups vs MI grou＇p. Plasma ET and P-selectin concentrations were also decreased markedly（ P 〈 0.05-0.01 ）, compared with MI group. Conclusion Yangxin granules could effectively protect vascular endothelial cells and myocardial injury in mongrel dogs with myocardial infarction.