CL-L 1基因在肝癌中的表达及其功能研究

Expression and function of CL-L 1 in hepatocellular carcinoma

目的:通过对肝癌相关基因CL-L1表达的研究,探讨其在肝癌发病机制中的作用。方法:采用RT-PCR分析CL-L1基因在61例肝癌和癌旁组织中的表达差异;利用pcDNA3.1/Myc-His(-)B和pEGFP-N1载体将CL-L1基因转入PCL肝癌细胞株,观察其克隆形成能力的变化以及该基因产物的亚细胞定位。结果:CL-L1基因在75.4%的肝癌组织中明显下调;该基因蛋白产物定位于细胞质中;CL-L1基因过表达引起PCL细胞的克隆形成能力明显下降。结论:CL-L1可能是一个新的肝癌相关的抑癌基因,对其进行深入研究有可能发现肝癌发病的新机制,也有可能发现肝癌治疗的新靶点。

Objective： To exptore further the mechanism of （HCC） via the study on CL-L 1 gene that is one of hepatocellalar carcinoma （HCC） related genes . Methods： Reverse transcriptase-polymerase chain reaction （RT-PCR） was performed to investigate the expression profile of CL-L 1 gene in 61 cases of HCC and compared with the corresponding adjacent non cancerous livers. The entire open reading frame （ORF） of human CL-L 1 gene was subcloned into mammalian cell expression vectors pcDNA3.1/Myc-His（-） B and pEGFP-N1 vectors, respectively. The vectors were transfected into HCC cell line PCL. The clony formation ability of PCL cells and subcellular localization of CL-L 1 gene were observed. Results： CL-L 1 gene was significantly downregulated in 75.4% （46/61） HCC tissues. CL-L 1 gene was localized in cytoplasm. Overexpression of CL-L 1 gene caused obvious decrease in the colony-formation ability of PCL cells. Conclusions： CL-L 1 is probably a potential tumor suppressor gene in HCC. It might be an important event in the oncogenesis of human HCC and may be a new target for HCC treatment.