高效液相色谱法同时测定姜参胶囊中人参皂苷Rg_1、Re的含量

Determination of Ginsenoside Rg1 and Ginsenoside Re in Jiangshen Capsules by HPLC Simultaneously

目的：建立以高效液相色谱法同时测定姜参胶囊中人参皂苷Rg1、Re含量的方法。方法：色谱柱为Kromasil C18，流动相为乙腈-0．05％磷酸溶液（21：79），检测波长为203nm，流速为1．0mL·min^-1。结果：人参皂苷Rg1、Re进样量分别在0．502～4．016μg（r=0．9997）、1．090-8．720μg范围内呈良好的线性关系（r=0．9998）；平均回收率分别为98．8％（RSD=1-24％）、99．4％（RSD=1．68％）。结论：本法操作简便、快速、准确可靠，可用于姜参胶囊的质量控制。

OBJECTIVE： To determine ginsenoside Rg1 and ginsenoside Re in Jiangshen Capsules by HPLC simultaneously. METHODS： The separation was performed on Kromasil - C18 column, the mobile phase consisted of acetonitrile - 0.05% H3PO4 solution （21 ： 79） with flow rate of 1.0mL · min^ -1 and detection wavelength of 203nm. RESULTS： The linear ranges of ginsenoside Rg1 and ginsenoside Re were 0.502- 4.016μg（ r ： 0.999 7） and 1.090- 8.720μg（ r = 0.999 8）, respectively, with average recovery at 98.8% （RSD= 1.24%） and 99.4% （RSD= 1.68%）, respectively. CONCLUSION： This method is simple, rapid, accurate and reliable, and suitable for the quality control of Jiangshen Capsules.