结肠癌中MMP-2和MMP-9蛋白表达与微血管计数的关系

Correlation of MMP-2 and MMP-9 protein expression on the angiogenesis of colonic carcinoma

目的探讨MMP-2、MMP-9蛋白在结肠肿瘤的血管形成中的临床意义。方法用免疫组化法检测31例结肠癌中MMP-2、MMP-9、VEGF及CD34相关抗原的蛋白表达情况,统计分析MMP蛋白与结肠肿瘤微血管密度的关系。以20例溃疡性结肠炎、21例结肠腺瘤和10例正常结肠粘膜作为对照组。结果结肠癌中MMP-2、MMP-9、VEGF蛋白表达阳性率和微血管密度计数(MVD)明显高于正常结肠粘膜组织(P<0.05),MMP-9各组间比较差异无统计学意义,从溃疡性结肠炎、结肠腺瘤到结肠癌中MMP-2、MMP-9、VEGF蛋白表达阳性率和MVD不同且具有递增趋势。MMP-2、MMP-9、VEGF蛋白表达和MVD与结肠癌的Duke’s分期及有无淋巴结转移显著相关(P<0.05)。结肠癌中MMP-2、MMP-9表达阳性者与MMP-2、MMP-9表达阴性者相比,VEGF蛋白表达阳性率和MVD显著不同(P<0.05)。结论MMP-2、MMP-9蛋白过度表达在结肠肿瘤的血管形成中具有重要的临床意义。

Aim To investigate the clinicopathological role of MMP-2 and MMP-9 on the angiogenesis of colonic carcinomal Methods MMP-2, MMP-9, VEGF and CD34 relative antigentic protein expression was measured by immunohistochemistry in resection specimens from 31 colonic carcinoma patients, 20 ulcerative colitis, 21 colon adenoma and 10 normal colon tissues as control respectively. Correlations between the MMP protein expression and the MVD counting were analysed. Results The positive rate of MMP-2 and VEGF protein expression and the MVD counting in colonic carcinoma was higher than that in normal colon tissues（ P 〈 0.05）, but the positive rate of MMP-9 protein expression in colonic carcinoma had no significant discrepancy with that in normal colon tissues, the MMP-2, MMP-9, VEGF protein expression and the MVD counting had significant discrepancy and increased between ulcerative colitis, colon adenoma and colonic carcinoma. The positive rate of MMP-2, MMP-9, VEGF protein expression and the MVD counting were significantly correlated with the clinicopathologic parameters such as the Dukes stage and the lymph nodes metastasis in colonic carcinoma（ P 〈 0.05）. The positive rate of VEGF protein expression and the MVD counting in MMP-2 or MMP-9 protein expression positive samples was higher than that in MMP-2 or MMP-9 protein expression negative samples （ P 〈 0.05）. Conclusion The overexpression of MMP-2 and MMP-9 protein contributes to the angiogenesis of colonic carcinoma.