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抗人膀胱癌单克隆抗体BDI-1的扩增和鉴定

The production and characterization of antihuman bladder carcinoma monoclonal antibody BDI-1
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摘要 目的制备纯化的抗人膀胱癌单克隆抗体BDI-1。方法给Balb/c小鼠腹腔注射BDI-1杂交瘤细胞,收集的腹水过Protein G-agaros亲和层析柱即得到人膀胱癌BDI-1单克隆抗体。采用ELISA法、间接免疫荧光法、SDS-聚丙烯酰胺凝胶电泳法和点印迹法鉴定单克隆抗体。结果每毫升腹水可以得到1-1.5mg的高效价(106)的纯化抗体。结论该方法可以扩增高效价的单克隆抗体. To make use of purified antihuman bladder carcinoma monoclonal antibody BDI-1. Balb/c mice was injected hybridoma BDI-1 in peritoneal cavity,then use Protein C-agros affinity chromatography rarefied ascitesthat had been got.So we gained anfihuman bladder carcinoma monoclonal antibody BDI-1. Methods antibody was characterized by ELISA , indirect immunofluoresence ,SDS-PAGE electrophoresis and dot blot.Result; It was got 1-1.5 mg high titer（106） and purified antibody every milliliter ascites. Conclusion This way may produce high titer monoclonal antibody.
作者 潘雪娜 叶炯贤 贾少微 高凤彤
机构地区 北京大学深圳医院核医学科 北京大学深圳医院泌尿外科 吉林大学中日联谊医院核医学科
出处 《中国实验诊断学》 2007年第2期146-148,共3页 Chinese Journal of Laboratory Diagnosis
基金 深圳市科技计划资助项目(200204116)
关键词 膀胱癌 BDI-1 单克隆抗体 bladder carcinoma BDI- 1 monoclonal antibody
分类号 R737.14 [医药卫生—肿瘤]
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参考文献5

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中国实验诊断学
2007年 第2期

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