摘要
目的通过检测胃癌Runx3基因启动子区域甲基化状态,来探讨Runx3基因启动子区域甲基化在胃癌发生和发展过程中的临床意义。方法采用DNA甲基化特异性PCR(MSP)技术对37例胃癌患者手术切除肿瘤组织及其癌旁组织Runx3基因启动子区域甲基化进行检测。结果Runx3基因在37例胃癌及其癌旁组织中甲基化率分别为40.5%(15/37)和8%(3/37)。结论Runx3基因启动子区甲基化可能是肿瘤特异性的(P<0.05),可作为胃癌早期诊断的分子标记物。
Objective To study the clinic significance of Runx3 hypermethylation in gastric cancer, we examined the promoter methylation status of these genes in both neoplastic and non-neoplastic gastric epithelia obtained at surgery.Methods The methylation status of Runx3 was examined by methylation-speeific polymerase chain reaction（MSP）. Results Among samples obtained from patients with stomach cancer,methylation was observed in both the neoplastic and the corresponding non-neoplastic gastric epithelia, 40.2 % （ 15/37 ） and 8 % （3/37） for Runx3, respectively. Conclusion High frequent hypennethylation of Runx3 is mostly cancer specific（ P 〈 0.05）, and may be utilized as a molecular diagnostic marker of gastric cancer.
出处
《中国实验诊断学》
2007年第2期152-154,共3页
Chinese Journal of Laboratory Diagnosis
