氟对体外培养成骨细胞氧化应激和增殖活性的影响

The effects of fluoride on oxidative stress and proliferative activity of osteoblasts

目的研究氧化应激反应对染氟成骨细胞增殖活性的作用。方法取昆明小鼠乳鼠颅骨进行成骨细胞培养,应用生化方法检测细胞内蛋白和丙二醛(MDA)含量及超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GPX)活性。结果暴露于低水平氟(0.5-1mgF-/L)的成骨细胞脂质过氧化物MDA水平明显较低,但在2 mgF-/L组MDA水平明显提高;长时间(≥72 h)暴露于高水平氟的成骨细胞MDA含量显著增多。染氟24-72 h的成骨细胞GPX活性在低氟(0.5-2.0 mgF-/L)组明显升高,暴露于高氟环境的成骨细胞酶活性下降。结论低浓度氟刺激成骨细胞增殖和氧化应激活跃;高浓度氟引起过量氧化应激和细胞增殖受抑制。

Objective To study the role of oxidative stress on proliverative activity in fluoride-treated esteoblasts.Methods onteoblasts isolated from calvaria of neonatal mice were cultured and treated with fluoride for 24 - 72 h. The biochemical methods were utilized to test the level of MDA and the activities of SOD and GPX of ostcoblasts. Results The levels of MDA showed lower in the osteoblasts treated with 0.5 - 1 mg/L of fluoride, but increased in osteoblasts treated with 2 mg/L of fluoride. The levels of MDA increased in osteoblasts exposed to high concentration d fluoride for 72 h. The activities of GPX were obviously higher in osteohlasts treated with 0.5- 2 mg/L of fluoride for 24- 72 h, but decreased in osteoblasts treated with high concentration of fluoride.Conclusion Low concentration of fluoride stimulated both active oxidative stress and high proliverative activity; high concentration of fluoride induced excessive oxidative stress but inhibited the growth of osteoblasts.