16SrRNA基因在诊断慢性非细菌性前列腺炎中的应用

Application of 16SrRNA gene for the diagnosis of chronic abacterial prostatitis

目的探索快速可靠的检测慢性非细菌性前列腺炎细菌感染的新方法。方法应用PCR方法检测70例慢性非细菌性前列腺炎患者的前列腺液和尿道拭子中细菌16SrRNA基因。用PCR技术扩增实验室保留株10株的16SrRNA基因,以人类基因组DNA、HBV-DNA和白色念珠菌为对照,检测该方法的特异性;采用倍比稀释法进行该方法的灵敏度检测。结果细菌16SrRNA基因的检出率在前列腺液中和尿道拭子中分别为54.3%和7.1%,差异有显著意义(P<0.01)。对所测细菌株均获得920 bp扩增产物,而与人基因组DNA、HBV-DNA和白色念珠菌无交叉反应;PCR最低能检测1.5*106/L大肠杆菌DNA。结论慢性非细菌性前列腺炎患者的前列腺液中有细菌16SrRNA基因的检出,其病因可能与细菌感染有关。PCR具有快速,特异性和敏感性高的特点。

Objective To study a rapid and reliable method for the detection of bacteria infective in chronic abacterial prostatitis.Methods PCR was used to detect bacterial 16SrRNA gene signal in EPSs and urethral swabs of 70 men with chronic abaeterial prostatitis; 16SrRNA gene of ten bacterial species were amplified with PCR, by using human genome DNA,HBV-DNA and candida albieans as comparison;The sensitivity test was done by the method of gradual dilution. Results Positive rate of bacterial 16SrRNA gene in EPSs and urethral swabs samples were 54.3% and 7.1% ,respectively（ P 〈 0.01）. The bacterial species were amplified and the products were 920 bp in length , but human genome DNA,HBV-DNA and candida albicans showed no amplification products. Sensitivity test showed that it could detect as low asl. 5^＊10^6/L of E. coli DNA. Conclusion Bacterial 16SrRNA gene signal had been detected in EPS of patients with chronic abacterial prostatitis. Bacterial infection is possible related to chrenic abacterial prostatitis.The method of PCR is rapid and highly specific and sensitive in detecting the existence of bacterial 16SrRNA gene.