生大黄防治新生鼠缺氧肠损伤的初步研究

Primary research on crude rhubarb preventing hypoxia-induced intestinal injury in neonatal rat model.

【目的】为寻找新生儿坏死性小肠结肠炎(necrotizing enterocolitis,NEC)更好的临床防治方法,对生大黄防止新生鼠缺氧性肠损伤的效果进行了观察。【方法】选用新生1日龄Wistar大鼠,将其制成缺氧肠损伤模型,然后喂生大黄(A组),4 d后取肠组织作病理评分检查,并检测肠组织中丙二醛(malondialdehyde,MDA)及肿瘤坏死因子(tumor necrosis factor-α,TNF-α)含量。同时设皮下注射肠三叶因子(B组)及对照组(C组为未给药组、D组为未行缺氧实验组)。【结果】AB两组动物肠组织病理损伤程度轻,病理评分相近,均低于C组(P<0.01)。A、B两组MDA及TNF-α含量低于C组,差异有非常显著性(P<0.01),与D组比较无差别(P>0.05)。【结论】生大黄具有与肠三叶因子相同的防治NEC的效果,能减少缺氧肠损伤组织中TNF-α及MDA的产生。因其价廉易得,且已在新生儿临床应用,故比肠三叶因子具有更好的应用前景。

[Objective} To find better method of preventing necrotizing enterocolitis（NEC）. [Methods] After NEC modeles of neonatal rats were established, fourty rats were derided into four groups. Group A：cured with crude rhubarb. GroupB; injected with inteslinal trefoil factor（ITF）. Group C： NEC but not be cured. Group D： not lo be NEC,norreal conlral. On the 4th day, intestinal tissues were obtained to do pathologic inspection and to determine lbe content of TNF-α and malondialolehyde （MDA）. [Results] The intestinal palhologic injury in group A and B was light, and pathologic scores in these groups were lower than that in group C（P〈0. 01）. Content of TNF a and MDA in group A and B was lower than those in group C（P〈0. 01）, was nearly similar with group D （P〈0. 05）. [Conclusions] Crude rhubarb has similar effect on preventing NEC with ITF, and could reduce the TNF-α and MDA in damaged intestinal tissue. It is also cheap and easily obtained, so will be better used in neonate clinical treatment.