不同浓度甲醛致小鼠肾细胞DNA损伤效应研究

Study on DNA damage of the kidney cells of mice induced by formaldehyde at different doses

为了探讨甲醛导致生物机体内的DNA损伤效应及其剂量-效应关系,通过不同浓度的液态甲醛对小鼠肾细胞进行染毒,并分别运用了单细胞凝胶电泳实验、荧光检测法实验和KCl-SDS沉淀法实验进行研究.结果显示,甲醛在低浓度(5μmo·lL-1)时,具有致DNA断裂的作用;在中等浓度(25μmo·lL-1)时,对DNA的损伤作用以DNA-DNA交联为主;在高浓度(125、625μmo·lL-1)时,对DNA的损伤作用以DNA-蛋白质交联为主.

Formaldehyde is one of the indoor air pollutions with the characteristics of extension sources and serious biological toxicities. The kidney cells of mice were chosen as materials to evaluate the relationship between the types of damage on DNA and different doses of liquid formaldehyde by the methods of single cell gel electrophoresis （SCGE）, fluorescent detection assay and KCl-SDS assay. The results demonstrated that formaldehyde could induce DNA strand breaks at the lower dose （5 μmol·L^-1）, and could primarily induce DNA-DNA crosslinks at the middle dose （25 μmol·L^-1）, as well as DNA-protein crosslinks at the higher dose （125,625μmol·L^-1）.