电磁脉冲照射对小鼠脑组织氧化状态的影响及姜黄素的治疗作用

Effect of Electromagnetic Radiation on Oxidation Balance of Mice Brain and Therapeutic Effect of Curcumin

目的观察电磁脉冲(EMR)照射后小鼠脑组织超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)的变化及姜黄素的防护作用。方法40只小鼠随机分为空白对照组、单纯EMR组、EMR+姜黄素低剂量(20mg/kg/d)组、中剂量(40mg/kg/d)组、高剂量(80mg/kg/d)组,每组8只。EMR组和EMR+姜黄素组小鼠接受200kV/m的EMR照射,EMR+姜黄素组小鼠同时每日给予不同剂量的姜黄素,5d后停止照射及给药,测定小鼠脑组织中SOD、GSH-Px、MDA的变化。结果与空白对照组比较,EMR照射组小鼠脑组织SOD与GSH-Px活性及MDA含量上升(均P<0.05);与单纯EMR组比较,EMR+姜黄素中、高剂量组小鼠脑组织SOD与GSH-Px活性及MDA含量下降(均P<0.05)。结论200kV/m的EMR照射可导致小鼠脑组织过氧化,姜黄素可通过其抗氧化作用有效治疗这种损伤,且效应呈一定的剂量依赖关系。

Objective To explore the changes of superoxide dismutase （SOD）, glutathione peroxidase （GSH-Px）, malondialdehyde （MDA） in the brain of mice after irradiated by electromagnetic radiation （EMR） and therapeutic effect of curcumin. Methods 40 mice were divided randomly into the bare control group, simply EMR group, EMR＋curcumin low dose, middle dose and high dose groups total 5 groups with 8 animals in each group. The mice except the bare control group received EMR irradiation and those in the EMR＋curcumin groups were given various doses of curcumin at the same time. Five days later, EMR irradiation and medication stopped, and the levels of SOD, GSH-Px and MDA in the brain of mice were tested. Results Compared with the bare control group, the activities of GSH-Px and SOD, and level of MDA all increased in the mice irradiated by EMR （ P 〈0.05）. Compared with the simply EMR group, the activities of GSH-Px and SOD, and level of MDA all decreased in the mice of EMR ＋curcurain groups （ P 〈0.05）. Conclusion EMR irradiation can induce changes of GSH-Px and SOD and peroxidation of mice brain, curcurain can lighten these damages by its anti-oxidation with a dose-dependent effect.